生物炭对不同酸化水平稻田土壤性质和重金属Cu、Cd有效性影响

针对南方稻田土壤酸化严重,导致养分流失有毒重金属活化,严重影响稻米质量安全的重大现实问题。以水稻秸秆和谷壳等农业废弃物为原料制备生物炭(分别记为RSC和RHC),研究不同原料生物炭对酸化土壤改良及其对重金属有效性的影响。设置3个生物炭用量(0,20,50 g/kg,分别记为CK、C1、C2),4种土壤酸化水平(pH 4.01,4.25,4.33,4.58,分别记为L1、L2、L3、L4),生物炭与重金属污染土壤共同培养60天后测定土壤pH、全氮、有机质、有效磷、速效钾和有效态Cu、Cd含量。结果表明:RSC对酸化土壤pH的改良效果明显优于RHC,且施炭量越高提高幅度越大,RSC的C2处理使4种酸度水平的土壤pH分别提高了0.68,0.97,1.29,1.71个单位。2种生物炭均能提高土壤的全氮、有效磷、速效钾和有机质含量,其中各施炭处理有机质显著提高,尤以速效钾的增幅最为显著,RSC对4种养分的提高均优于RHC。RHC对土壤有效态Cu含量无显著影响;RSC的C2较C1处理更能降低土壤中有效态Cu含量,使4种酸度水平的土壤分别降低了13.62%,6.57%,4.36%,7.88%。RHC处理的L3、L4土壤中有效态Cd含量显著降低,最大分别降低了13.79%,19.23%。RSC使4种酸度土壤有效态Cd含量最大分别降低了20.00%,25.81%,20.69%,19.23%。相关分析表明,土壤pH与有效态重金属含量呈显著负相关关系。水稻秸秆炭用于改良酸化土壤、降低重金属Cu和Cd有效性的效果更佳,且降低污染土壤中Cd的有效性较Cu好;生物炭对酸化程度越低的土壤pH和有效磷含量的提高以及有效态Cd含量的降低效果较好,而有效态Cu含量的降低效果则在酸化程度越高的土壤中表现更佳;土壤pH是生物炭调控重金属Cu、Cd有效性的主要影响因素。     

Quantitative trait loci mapping for yield-related traits under low and high planting densities in maize (Zea mays)

Average maize yield per hectare has increased significantly because of the improvement in high-density tolerance, but little attention has been paid to the genetic mechanism of grain yield response to high planting density. Here, we used a population of 301 recombinant inbred lines (RILs) derived from the cross YE478 × 08–641 to detect quantitative trait loci (QTLs) for 16 yield-related traits under two planting densities (57,000 and 114,000 plants per ha) across four environments. These yield-related traits responded differently to high-density stress. A total of 110 QTLs were observed for these traits: 33 QTLs only under low planting density, 50 QTLs under high planting density and 27 QTLs across both densities. Only two major QTLs, qCD6 and qWKEL2-2, were identified across low- and high-density treatments. Seven environmentally stable QTLs were also observed containing qED6, qWKEL3, qRN3-3, qRN7-2, qRN9-2 and qRN10 across both densities, as well as qRN9-1 under low density. In addition, 16 and eight pairs of loci with epistasis interaction (EPI) were detected under low and high planting densities, respectively. Additionally, nine and 17 loci showed QTL × environment interaction (QEI) under low- and high-density conditions, respectively. These interactions are of lesser importance than the main QTL effects. We also observed 26 pleiotropic QTL clusters, and the hotspot region 3.08 concentrated nine QTLs, suggesting its great importance for maize yield. These findings suggested that multiple minor QTLs, loci with EPI and QEI, pleiotropy and the complex network of “crosstalk” among them for yield-related traits were greatly influenced by plant density, which increases our understanding of the genetic mechanism of yield-related traits for high-density tolerance.     

How does water and salt stress affect the germination and initial growth of Brazilian soya bean cultivars?

The effects of water and salt stress on rate of germination and seedling growth were investigated under laboratory conditions in 46 soya bean genotypes from Central-West region of Brazil to verify how these stresses may limit crop establishment during the initial growth stage and also to identify the most tolerant genotypes to drought and salinity. Mild water and salt stresses were imposed by seed exposure to –0.20 MPa iso-osmotic solutions with polyethylene glycol—PEG 6000 (119.57 g/L) or NaCl (2.357 g/L) for 12 days at 25°C. The germination percentage, seedling length and seedling dry matter were measured, and then, salt or drought tolerance indexes were calculated. The “NS 5909 RG,” “NS 7000 IPRO,” “NS 7338IPRO,” “FPS Solimões RR,” “NS 5151 IPRO,” “SYN 13610 IPRO,” “LG 60177 IPRO,” “NS 6909 IPRO” and “BMX Desafio RR” were identified as the most drought-tolerant genotypes, whereas under salinity conditions, the genotypes “5D 615 RR,” “BMX Desafio RR,” “5D 6215 IPRO” and “BMX Ponta IPRO” were identified as tolerant. The “BMX Desafio RR” is the genotype most adapted to both stress conditions and, therefore, should be used under conditions of water shortage and excess salt in the soil at sowing time.     

Rapid mapping of candidate genes for cold tolerance in Oryza rufipogon Griff. by QTL-seq of seedlings

Cold stress is a major problem in rice production. To rapidly identify genes for cold tolerance in Dongxiang wild rice(DWR, Oryza rufipogon Griff.), sequencing-based bulked segregant analysis of QTL-seq method was used to resequence the extremely resistant(R) and susceptible(S) bulks of a backcross inbred lines(BILs) population(derived from Oryza sativa×O. rufipogon) and their parents. Single nucleotide polymorphisms(SNP)-index graphs and corresponding Δ(SNPindex) graphs(at 99 and 95% confidence levels) for R-and S-bulks detected a total of 2 609 candidate SNPs, including 58 candidate cold-tolerance genes. Quantitative real-time PCR analysis revealed that 5 out of the 58 candidate genes had significant differences in expression between O. sativa and O. rufipogon. Structural variation and functional annotations of the 5 candidate genes were also analyzed, and allowed us to identify 2 insertion-deletion(InDel) markers(12-7 and 12-16) that were linked with candidate genes on chromosome 12 in DWR. These results are helpful for cloning and using cold tolerance genes from common wild rice in cultivated rice.     

Selection of soybean elite cultivars based on phenotypic and genomic characters related to lodging tolerance

Soybean lodging can result in serious yield reduction. Detecting the quantitative trait loci (QTL) associated with lodging tolerance for their further application in marker‐assisted selection (MAS) has the potential to enhance soybean breeding efficiency. In this study, a genome‐wide association analysis (GWAS) was performed to identify soybean accessions that could potentially be used to produce lodging‐tolerant varieties, based on the comprehensive evaluation of lodging scores (LS) obtained for the parental cultivar “Tokachi nagaha” and its 137 derived cultivars. Results showed that genotype, environment and genotype × environment interaction significantly influenced LS. Of the 31 significant SNPs identified, 22 were consistently detected in two or more environments and 27 SNPs were located in or close to agronomically important QTL mapped by linkage analysis. Best linear unbiased predictors (BLUPs) of LS tend to decrease with the elite alleles contained by accessions increasing. Some excellent accessions, with lower BLUPs and D_i (stability coefficients) values and more elite alleles, were selected. This study contributed to understand the genetic mechanism of lodging, providing genetic and phenotypic information for MAS.     

益生菌大肠杆菌Nissle 1917抗逆性能、猪肠上皮细胞黏附率及抑菌效果研究

本试验旨在研究益生菌大肠杆菌Nissle 1917(Ec N)抗逆性能、猪肠上皮细胞黏附率及抑菌效果。采用体外法对Ec N进行生长曲线绘制和耐酸、耐胆盐、耐热性能的测定;以猪肠上皮细胞IPEC-J2细胞为体外细胞模型,考察了Ec N对该细胞的黏附率以及对致病菌大肠杆菌K88的黏附抑制率;同时通过蛋白质印迹法检测了Ec N对IPEC-J2细胞β-防御素-2和Toll样受体4的水平的影响。结果表明:1)Ec N对高酸、高胆盐和高温环境具有一定耐受能力。2)Ec N对IPEC-J2细胞的黏附作用以对数期最佳,黏附率达33.96%,显著高于迟缓期、稳定期和衰亡期(P0.05)。3)Ec N对致病菌大肠杆菌K88具有良好的抑制效果,黏附抑制率达87.84%。4)Ec N还能上调IPEC-J2细胞β-防御素-2和Toll样受体4水平。结果提示,益生菌Ec N具有较好的抗逆性能,能够良好地黏附猪肠上皮细胞,对致病菌大肠杆菌K88具有良好的抑制作用。     

解析基本偏差系列图的妙用

为了使机械产品在激烈竞争的市场上立稳脚跟并得到迅猛发展,需要进行高度专业化协作性生产,这就要求组成机械产品的零或部件具有互换性.所以无论是在读的机械大类的学生,还是从事机械行业的技术工人、工程师,巧用基本偏差系列图,不仅妙趣横生,有时还会事半功倍.     

Differences in Ion Accumulation and Salt Tolerance among Glycine Accessions

Salinity reduces crop yield by limiting water uptake and causing ion‐specific stress. Soybean [Glycine max (L.) Merr.] is sensitive to soil salinity. However, there is variability among soybean genotypes and wild relatives for salt tolerance, suggesting that genetic improvement may be possible. The objective of this study was to identify differences in salt tolerance based on ion accumulation in leaves, stems and roots among accessions of four Glycine species. Four NaCl treatments, 0, 50, 75 and 100 mm , were imposed on G. max, G. soja, G. tomentella and G. argyrea accessions with different levels of salinity tolerance. Tolerant genotypes had less leaf scorch and a greater capacity to prevent Na⁺ and Cl^? transport from soil solution to stems and leaves than sensitive genotypes. Magnitude of leaf injury per unit increase in leaf Na⁺ or Cl^? concentrations was lower in tolerant than in susceptible accessions. Also, plant injury was associated more with Na⁺ rather than with Cl^? concentration in leaves. Salt‐tolerant accessions had greater leaf chlorophyll‐meter readings than sensitive genotypes at all NaCl concentrations. Glycine argyrea and G. tomentella accessions possessed higher salt tolerance than G. soja and G. max genotypes.     

绿豆品种资源萌发期耐碱性鉴定

采用人工气候箱内培养皿培养,以混合碱Na HCO3∶Na2CO3(摩尔比)为9∶1模拟典型东北地区碱胁迫环境,在萌发期以50 mmol L–1溶液处理34份绿豆品种资源,蒸馏水处理为对照,每培养皿放30粒种子。培养第3天测定发芽势,第7天测定发芽率、下胚轴长、胚根长、下胚轴干重、胚根干重等指标,通过隶属函数法和聚类分析对参试材料耐碱性综合评价,并进行因子分析。利用隶属函数法对参试材料耐碱性排序表明,不同绿豆品种资源间表现出较大差异,聚类分析把参试材料按耐碱性强弱分为4大类,白绿11等9份材料为耐碱类型,公绿1号等19份材料为耐碱中间类型,吉绿3号等5份材料为碱敏感类型,潍绿7号为碱极敏感类型。因子分析结果表明,萌发指数、下胚轴干重、胚根长分别在萌发因子、生物量累积因子和伸长因子中的负荷量最大,可作为绿豆萌发期耐碱性鉴定的适宜指标。     

遮荫对10种地被植物光合特性的影响

为了探讨地被植物在不同遮荫条件下的适应性,本研究以10种地被植物为材料,对其在不同光照条件下的生长状态、日平均净光合速率、表观量子效率及光补偿点进行分析.结果表明:遮荫水平对10种植物的光合有很大影响,10种植物能忍受的遮荫程度不同.对旋覆花、甘野菊、大叶铁线莲、青杞、委陵菜来说,全光比较合适；对蛇莓、匍枝毛茛、匍枝委...